![]() ![]() Of a protease is the characterization of the respective natural substrate. These rules were verified by checking against information publishedĮstablishing a specificity model for protease cleavage:Ī first and obvious approach to obtain information concerning the cleavage specificity Specificity were adopted from the comprehensive publication of Keil Likewise, the residues in C-terminal direction are designated P1', P2', in the N-terminal direction from the cleavedīond. ![]() Subsite nomenclature was adopted from a scheme created by SchechterĪnd Berger (1967, 1968) and used in the following description of enzyme specificities.Īccording to this model, amino acid residues in a substrate undergoing cleavageĪre designated P1,P2, P3, P4 etc. The cleavage specificities of selected enzymes and chemicals:
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